Table 1 PCR primers used to detect C. jejuni and C. coli in stool samples
From: Epidemiology of Campylobacter infections among children of 0–24 months of age in South Africa
Primer name | Organism detected | Gene name and size (bp) | Sequence (5’-3’) and annealing temperature | References |
|---|---|---|---|---|
Hip 1a Hip 2b | C. jejuni | Hippuricase gene, 176 | -ATG ATG GCT TCT TCG GAT AG- -GCT CCT ATG CTT ACA ACT GC- 58 °C | Linton et al. [14] |
CC18F CC519R | C. coli | Aspartokinase gene and the downstream ORF of C. coli, 500 | -GGT ATG ATT TCT ACA AAG CGA G- -ATA AAA GAC TAT CGT CGC GTG- 66 °C | Linton et al. [14] |