Volume 73 Supplement 1

Methods in Epidemiology Symposium

Open Access

Changes in miRNA expression and retinal blood vessels are associated with short-term air pollution exposure.

  • Tijs Louwies1,
  • Luc Int Panis2,
  • Patrick De Boever2 and
  • Tim Nawrot1
Archives of Public HealthThe official journal of the Belgian Public Health Association201573(Suppl 1):P24

https://doi.org/10.1186/2049-3258-73-S1-P24

Published: 17 September 2015

Air pollution, a cardiovascular risk factor, might exert its effects through the microcirculation. Fundus photography allows study of the retinal vasculature in vivo. Short-term PM10 exposure is associated with changes in retinal blood vessels, but the underlying mechanism remains unknown. Expression of MIR21, MIR222 and MIR146A, gene regulators involved in oxidative stress and inflammatory processes, can be changed by air pollution and might be a pathway explaining the association between PM10 and microvascular changes.

50 healthy adults (50% women, 50% men, 23-58 years old) were sampled once a month from December 2014 until April 2015. At each study visit fundus photos and venous blood samples were collected. PM10 data were obtained from a nearby monitoring station. Image analysis was used to calculate the width of retinal blood vessels, represented as the Central Retinal Arteriolar/Venular Equivalent (CRAE/CRVE). miRNA was isolated from blood and expression was measured using qRT-PCR. Mixed models were used for statistical analysis.

Short-term changes in PM10 exposure were associated with changes in CRAE, CRVE and miRNA-expression. Each 10 μg/m3 increase in PM10 during the previous 24 hours was associated with a 0.58 μm decrease (95% CI: -1.16, -0.0005; p=0.056) in CRAE, a 0.99 μm increase (95% CI: 0.18, 1.80; p=0.021) in CRVE, a 6.6% decrease (95% CI: -11.07, -2.17; p=0.0038) in miR-21 expression and a 6.7% decrease (95% CI: -10.70, -2.75; p=0.0012) in miR-222 expression. miRNA expression was associated with CRAE and CRVE. Each 10% increase in miR-21 expression and miR-222 was associated with respectively a 0.14 μm increase (95% CI: 0.0060, 0.24; p=0.046) in CRAE and a 0.28 μm decrease (95% CI: -0.50, -0.062; p=0.016) in CRVE.

PM10 exposure affects miRNAs involved in inflammation and oxidative stress. These changes may be an underlying mechanism for the association between PM10 exposure and retinal arteriolar narrowing and venular widening.

Authors’ Affiliations

(1)
Hasselt University
(2)
Flemish Institute for Technological Research (VITO)

Copyright

© Louwies et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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